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Journal of Integrative Neuroscience  2019, Vol. 18 Issue (3): 237-243    DOI: 10.31083/j.jin.2019.03.1100
Original Research Previous articles | Next articles
Effects of low-frequency repetitive transcranial magnetic stimulation on depression- and anxiety-like behaviors in epileptic rats
Shun Wang1, Shanping Mao2, *(), Baozhen Yao1, *(), Dan Xiang3, Congcong Fang1
1 Department of Pediatrics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P. R. China
2 Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P. R. China
3 Department of Psychiatry, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P. R. China
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Low-frequency repetitive transcranial magnetic stimulation (rTMS) has been considered as a treatment option for depression and anxiety. However, its role in epilepsy comorbid with depression and anxiety is unclear. Therefore, we evaluated whether low-frequency rTMS can alleviate depression- and anxiety-like behavior in epileptic rats. Forty-eight adult rats were allocated at random to four groups: Control, Pentylenetetrazol (PTZ), PTZ-rTMS and PTZ-Sham. The control group received intraperitoneal injections of normal saline, while the other groups received intraperitoneal injections of pentylenetetrazol (35 mg/kg/d) once a day for 15 days. Low-frequency rTMS or sham stimulation were administered to the PTZ-rTMS and PTZ-Sham group, respectively, over the two-week period. The open-field test (OFT), elevated plus-maze test (EPM) and forced swimming test (FST) were carried out before the experiment, on the 8th and 15th day to assess depression- and anxiety-like behavior in the rats. Two weeks of low-frequency rTMS treatment could not impair the increases of seizure severity in epileptic rats. However, relative to the PTZ and PTZ-Sham group, the two-week low-frequency rTMS treatment significantly reduced the immobility time in the forced swimming test and attenuated the progressive decrease in total distance traveled, frequency of rearing, velocity in the open-field test, number of entries in the open arms (%) and the time spent in the open arms (%) in the elevated plus-maze test of the PTZ-rTMS group. We proposed that low-frequency rTMS can benefit epileptic rats via amelioration of comorbid depression and anxiety, but it can not alleviate the seizure severity.

Key words:  Epilepsy      low-frequency rTMS      depression      anxiety      rat     
Submitted:  01 July 2019      Accepted:  23 August 2019      Published:  30 September 2019     
  • TFLC2018001/Medical Science Advancement Program of Wuhan University
*Corresponding Author(s):  Shanping Mao Email:; Baozhen Yao Email:   

Cite this article: 

Shun Wang, Shanping Mao, Baozhen Yao, Dan Xiang, Congcong Fang. Effects of low-frequency repetitive transcranial magnetic stimulation on depression- and anxiety-like behaviors in epileptic rats. Journal of Integrative Neuroscience, 2019, 18(3): 237-243.

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